SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

Spilki,Fernando Rosado; Franco,Ana Cláudia; Roehe,Paulo Michel.

Specific IgM, IgA, IgG1, IgG2, as well as neutralizing antibody responses were evaluated in sera of calves experimentally infected with two isolates of bovine herpesvirus type 1 (BoHV1) of distinct subtypes (subtype 1, BoHV1.1; subtype 2a, BoHV-1.2a). No significant differences were observed in the antibody responses induced by each BoHV-1 subtype. The antibody responses following primary acute infection were characterized by an increase in specific IgM and IgA levels between days 2 and 14 post inoculation (pi). IgG1 was detected from days 11 to 30 pi. IgG2 was detected on the sample taken on day 30 pi. Reactivation of infection following dexamethasone administration induced a significant rise in IgA levels, whereas IgG1 and IgG2 levels, which were at high...

Antibody responses in mice after immunization with inactivated bovine respiratory syncytial virus using different adjuvants

Palavras-chave: Bovine herpesvirus type 1; Infectious bovine rhinotracheitis; Immunoglobulin subclasses; ELISA.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200021

Spilki,Fernando Rosado; Almeida,Renata Servan de; Arns,Clarice Weis.

Bovine respiratory syncytial virus (BRSV) causes pneumonia in young cattle. Modified-live-virus (MLV) and inactivated vaccines are currently used for the control of clinical effects of BRSV infections in cattle. On the present research, the stimulation of specific anti-BRSV immunoglobulin isotypes was investigated, through the use of different commercially available adjuvants (Water-in-oil emulsion, Quil A, Aluminum-hydroxide) in inbred mice (Balb/C and C57BL/6). BRSV antibodies were measured using an enzyme-linked immunosorbent assay (ELISA) and the results were compared to the antibody levels induced by immunization of animals using live-BRSV-virus. Water-in-oil emulsion and alum- adjuvant preparations induced higher levels of IgG1 immunoglobulins,...

Anticorpos neutralizantes contra os vírus da cinomose e da parainfluenza em cães de canis dos municípios de Novo Hamburgo e Porto Alegre, RS, Brasil

Palavras-chave: Bovine respiratory syncytial virus; BRSV; Adjuvants; Murine model; Inactivated vaccines.

Ano: 2010

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010001100013

Hartmann,Tamahine Larronda Schmidt; Batista,Helena Beatriz de Carvalho Ruthner; Dezen,Diógenes; Spilki,Fernando Rosado; Franco,Ana Claudia; Roehe,Paulo Michel.

No presente estudo, foi realizada uma pesquisa em busca de anticorpos neutralizantes contra os vírus da cinomose (CDV) e da parainfluenza (CPIV) caninos em amostras de soro de 173 cães recolhidos a canis municipais em Novo Hamburgo (n=82) e Porto Alegre (n=91), RS. A pesquisa de anticorpos neutralizantes foi realizada pela técnica de soroneutralização frente a duas amostras vacinais de CDV (Rockborn e Snyder Hill) e frente a uma amostra de CPIV (V660). Em relação ao CDV, 95,9% das amostras de soros foram negativas para anticorpos neutralizantes contra a amostra Snyder Hill e 90,7% soronegativas para a amostra Rockborn. Entre os soropositivos (n=20; 11,6%), somente três deles apresentaram anticorpos neutralizantes frente às duas amostras de CDV testadas,...

Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A

Palavras-chave: Cinomose; Parainfluenza canino; Cães; Soroneutralização; Prevalência.

Ano: 2007

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782007000400045

Ferreira,Helena Lage; Spilki,Fernando Rosado; Santos,Márcia Mercês Aparecida Bianchi dos; Almeida,Renata Servan de; Arns,Clarice Weis.

Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV. The aim of the present study was to compare the detection of six subgroup A AMPV isolates (AMPV/A) viral RNA by using different conventional and real time RT-PCR methods. Two new RT-PCR tests and two real time RT-PCR tests, both detecting fusion (F) gene and nucleocapsid (N) gene were compared with an established test for the attachment (G) gene. All the RT-PCR tested assays were able to detect the AMPV/A. The lower detection limits were observed using the N-, F- based RRT-PCR and F-based conventional RT-PCR (10(0.3) to 10¹ TCID50 mL-1). The present study suggests that the...

Detecção molecular de vírus da bronquite infecciosa em plantéis de avós, matrizes e frangos de corte no Rio Grande do Sul e Mato Grosso

Palavras-chave: Avian metapneumovirus G; F; N genes real time RT-PCR RT-PCR.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000500022

Nascimento,Bárbara; Comerlato,Juliana; Spilki,Fernando Rosado.

O vírus da Bronquite Infecciosa das galinhas (VBI) pertence ao grupo 3 da família Coronaviridae e é o causador de desordens respiratórias e renais em frangos de corte. A vacinação com vacinas vivas é praticada em matrizes e avós e muitas vezes também nos plantéis destinados ao abate. As vacinas utilizadas no Brasil são usualmente do sorogrupo Massachusetts e baseadas nas amostras H120 e H52. É comum que após a vacinação o vírus vacinal seja detectado por isolamento em ovos embrionados ou por métodos moleculares por até 4 semanas. Após essa data, normalmente, não há detecção de vírus e o VBI, quando encontrado, pode representar recirculação do vírus vacinal no plantel ou a introdução de uma nova cepa do vírus. No presente estudo, para avaliar a circulação...

Detection of human adenovirus, rotavirus and enterovirus in water samples collected on dairy farms from Tenente Portela, Northwest of Rio Grande do Sul, Brazil

Palavras-chave: Bronquite infecciosa das galinhas; VBI; Nested-PCR; Avós; Matrizes.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000300016

Spilki,Fernando Rosado; Luz,Roger Bordin da; Fabres,Rafael Bandeira; Soliman,Mayra Cristina; Kluge,Mariana; Fleck,Juliane Deise; Rodrigues,Manoela Tressoldi; Comerlato,Juliana; Cenci,Alexander; Cerva,Cristine; Dasso,Maurício Gautério; Roehe,Paulo Michel.

Viral gastroenteritis and other waterborne diseases are a major concern for health in Brazil. A number of studies were conducted about the presence of viruses on water samples from Brazilian areas. However, the knowledge about the occurrence of viral contamination of drinking water sources in rural settings of the country is insufficient. On the present work, 15 samples from 5 dairy farms located at the municipality of Tenente Portela were collected and analysed for the presence of human adenoviruses (HAdV), as well as human enteroviruses (EV) and rotaviruses (RV). HAdV was present on 66.66% of the water samples, and have been found in all samples from artesian wells and springs, which are used as sources of drinking water for the individuals inhabiting...

Genotypes and clinical data of respiratory syncytial virus and metapneumovirus in brazilian infants: a new perspective

Palavras-chave: Human adenovirus; Water quality; Dairy farms.

Ano: 2013

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300046

BJID

Riccetto,Adriana Gut Lopes; Silva,Luciana Helena Antoniassi da; Spilki,Fernando Rosado; Morcillo,André Moreno; Arns,Clarice Weis; Baracat,Emílio Carlos Elias.

The aim of this study was to determine if there was a correlation between respiratory syncytial virus (RSV) and metapneumovirus (MPV) genotypes and clinical data of Brazilian infants hospitalized for acute lower respiratory infection. The viruses in the patients' nasopharyngeal secretions were studied using the polymerase chain reaction and phylogenetic analysis. The study assessed 144 infants; 31.9% were RSV positive and 5.6% were MPV positive. Statistical analysis was performed using the chi-squared test, Fisher's test, Odds ratio, univariate logistic regression, non-conditional multivariate logistic regression and the forward - stepwise method. Multivariate analysis confirmed a significant relationship between a positive PCR test for RSV and...

Isolamento do vírus Parainfluenza bovino tipo 3 no Rio Grande do Sul, Brasil

Palavras-chave: Respiratory syncytial virus; Metapneumovirus; Genotypes; Infants; Clinical.

Ano: 2009

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000100008

Gonçalves,Diocela Andrade; Spilki,Fernando Rosado; Chiminazzo,Cláudio; Oliveira,Michelle Andrade; Franco,Ana Cláudia; Roehe,Paulo Michel.

É descrito o isolamento do vírus Parainfluenza bovino tipo 3 (bPI-3) a partir de secreções nasais coletadas de um bovino com infecção respiratória. A identificação do agente foi realizada através de isolamento em cultivo celular e confirmada por testes de hemaglutinação, inibição da hemaglutinação, hemadsorção e imunofluorescência direta. Este é o primeiro registro do isolamento do vírus no Rio Grande do Sul.

Phylogenetic characterization of bovine parainfluenza 3 from contaminated cell cultures and field isolates from Brazil

Palavras-chave: Parainfluenza bovina; BPI-3.

Ano: 2003

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782003000500025

Vaucher,Rodrigo de Almeida; Dezen,Diogenes; Simonetti,Amauri Braga; Spilki,Fernando Rosado; Roehe,Paulo Michel.

Genomic fragments of the HN and L genes from Brazilian bovine parainfluenza 3 virus (bPIV-3) isolated as contaminants from cell cultures and clinical specimens were amplified by reverse transcription-polymerase chain reaction (RT-PCR), sequenced using specific degenerate primers and analyzed by phylogenetic comparison with reference strains of bPI3V. The Brazilian isolates revealed a high degree of genomic when compared to SF4/32 prototype strain, within the recently proposed genotype A of bPIV-3.

Seasonal variation on the presence of adenoviruses in stools from non-diarrheic patients

Palavras-chave: Bovine parainfluenza virus type 3; BPIV-3; Phylogenetic analysis.

Ano: 2011

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400029

Vetter,Michele Regina; Staggemeier,Rodrigo; Vecchia,Andréia Dalla; Henzel,Andréia; Rigotto,Caroline; Spilki,Fernando Rosado.

Human adenoviruses (HAdV), members of the Adenoviridae family, are excreted through the fecal route and may be present in the feces of humans consuming contaminated food or water. The presence of HAdV from different serotypes in the feces of healthy individuals was already reported using conventional polymerase chain reaction; however, real-time PCR (qPCR) may reveal not only the rates of detection as well as demonstrate the viral loads excreted by healthy persons. Aiming to identify and characterize the presence of adenoviruses in stool samples, 147 fecal samples from patients with no records of diarrhea were analyzed (74 from winter season and 73 from summer) by Real-Time PCR (qPCR) assay and conventional PCR. HAdV genome was present in 43.8% (32/73) of...

Seroprevalence of Hepatitis B and C markers at the population level in the municipality of Caxias do Sul, southern Brazil

Palavras-chave: HAdV; Stool samples; QPCR; Healthy individuals.

Ano: 2015

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300749

Menegol,Débora; Spilki,Fernando Rosado.

Chronic viral hepatitis are main public health problems worldwide. Data about the seroprevalence to Hepatitis B and C viruses (HBV e HCV) at the population level are scarce on Brazil and especially for the Southern region of the country. The seroprevalence to HBV and HBC antigens was evaluated on a large portion of the population of the municipality of Caxias do Sul (427,858 inhabitants), Brazil. A total of 60,604 individual serum samples collected from 2008 to 2011 were screened for HBV surface antigen (HBsAg) and for antibodies against HBsAg; anti HCV antibodies were measured by ELISA (Enzyme Linked Immunosorbent Assay). Overall, 1.63% of the individuals were positive for HBsAg and 1.43% showed seropositivity to HCV. From the total, 31,749 samples were...